DNA

Part:BBa_K2100041

Designed by: Wangui Mbuguiro   Group: iGEM16_MIT   (2016-10-17)


pEXPR pTALER21 - eYFP

This construct is the result of a gateway combining a TALER21 repressible promoter upstream of the eYFP gene, a yellow fluorescent protein. pTALER21 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TALER21 repressor inhibits the activator and transcription machinery from transcribing the eYFP gene.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 157
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
    Illegal BamHI site found at 311
    Illegal XhoI site found at 248
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 157
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 157
    Illegal PstI site found at 582
    Illegal AgeI site found at 365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 326


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